Mass spectrometric-based protein conformational analysis

Chemical crosslinking mass spectrometry

1. Research Projects

NSCF (81703471); Natural Science Foundation of Jiangsu Province (BK20170740)

2. Representative Research Achievements

(1) Temporo-spatial analysis of protein conformation

Transient crosslinking mass spectrometry enables the capture of protein intermediate conformers and high-resolution structural analysis. Time-resolved crosslinking strategy is developed for sequential analysis of protein conformational transitions.

(2) Drug target discovery-screening-binding mode evaluation

Development of native-denatured exchange mass spectrometry acquisition approach enables the identification of specific ligand-protein interactions. The coupling with chromatographic separation expands the application for probing specific ligand-protein interactions in biological matrices. A Fe₃O₄ nanoparticle-based approach is developed for drug-target screening in biofluids, which is beneficial for capturing target proteins with low abundance and greatly enhances the sensitivity and accuracy of target discovery.

1.Zhu, M.L.(#); Lu, K.G.(#); Jin, Y.T.; Xu, X.W.; Chu, C.Y.; Hao, H.P.*; Zheng, Q.L.*. Boronic derivatization-based strategy for monoacylglycerol identification, isomer annotation and quantification. Anal. Chim. Acta, 2022,1190,339233. (IF: 6.9)

2.Xia, D.D.; Liu, B.L.; Xu, X.W.; Ding, Y.*; Zheng, Q.L.*. Drug target discovery by magnetic nanoparticles coupled mass spectrometry. J Pharm Anal, 11 (2021) 122-127. (IF: 14.0)

3.Hou, Y.L.; He, D.D.; Ye, L.; Wang, G.J.*; Zheng, Q.L.*; Hao, H.P.*. An improved detection and identification strategy for untargeted metabolomics based on UPLC-MS. J. Pharm. Biomed. Anal., 191 (2020), 113531. (IF:3.2)

4.Hu, J.J.; Zheng, Q.L.*. Applications of mass spectrometry in the onset of amyloid fibril formation: focus on the analysis of early-stage oligomers. Front. Chem., 2020, 8:324. (IF: 3.69)

5.Zhu, M.L.(#); Xu, X.W.(#); Hou, Y.L.; Han, J.L.; Wang, J.; Zheng, Q.L.*; Hao, H.P.*. Boronic derivatization of monoacylglycerol and monitoring in biofluids. Anal. Chem., 91 (2019), 6724-6729. (IF:6.3)

6.Zheng, Q.L. (#); Ruan, X.J.(#); Tian Y.; Hu, J.J.; Wan, N.; Lu, W.J.; Xu, X.W.; Wang, G.J.; Hao, H.P.*; Ye, H.*. Ligand–protein target screening from cell matrices using reactive desorption electrospray ionization-mass spectrometry via a native-denatured exchange approach. Analyst, 144 (2019), 512. (IF:3.9)

7.Zheng, Q.L. (#); Tian Y.(#); Ruan, X.J.; Chen, H.; Wu, X.X.; Xu, X.W.; Wang, G.J.; Hao, H.P.*; Ye, H.*. Probing specific ligand-protein interactions by native-denatured exchange mass spectrometry. Anal. Chim. Acta, 1036 (2018), 58-65. (IF:5.1)

8.Zheng, Q.L.; Chen, H.*. Development and Application of Liquid Sample Desorption Electrospray Ionization Mass Spectrometry (LS-DESI-MS). Annu. Rev. Anal. Chem., 2016, 9, 411-448.(IF:8.0)

9.Zheng, Q.L.; Liu, Y.*; Chen, Q.H.*; Hu, M.H.; Helmy, R..; Sherer, C. E.; Welch, J. C.; Chen, H.*. Capture of Reactive Monophosphine-Ligated Palladium(0) Intermediates by Mass Spectrometry. J. Am. Chem. Soc. 2015, 137, 14035-14038.(IF:12.1)

10.Zheng, Q.L.; Zhang, H.*; Tong, L.Y.; Wu, S.Y.; Chen, H.*. Cross-Linking Electrochemical Mass Spectrometry for Probing Protein Three-Dimensional Structures. Anal. Chem. 2014, 86 (18), 8983-8991.(IF:5.8)