1. Phytochemical analysis and quality assessment of TCM based on modern instruments i.e. HPLC, LC-QqQ-MS, LCMS-IT-TOF et al;

2. The inherent mechanism study of TCM based on pharmacokinetics. Especially the mechanism study of deintoxication and/or synergism for TCM in the context of drug metabolite enzyme and transport protein.

1. Research Projects

(1)Qing Lan Project Outstanding Young Teachers of Jiangsu Province, 2017, ￥40,000.

(2)The Natural Science Foundation of Jiangsu Province, “Metabolic study of saikosaponins in the context of and combinatorial metabolism” (BK20161456), July 2016-July 2019, ￥100,000.

(3)The National Natural Science Foundation of China (NSFC), “A study of rhubaub-scutellaria compatibility in the context of Drug metabolite enzyme and transport protein involved in enterohepatic circulation” (81403314), Jan 2015-Dec 2017, ￥230,000.

(4)the Open Project Program of Guangxi Key Laboratory of Tradtitonal Chinese Medicaine Quality Stardands, “Combination of liquid-chromatography tandem mass spectrometry in different scan modes with biological samples for the study of Bupleurum saponins metabolism and pharmacokinetics”(201503) , Sept 2015-Sept 2017, ￥50,000.

(5)The Natural Science Foundation of Jiangsu Province, “Studies on the material base for the discrepancy in therapeutic effect between crude and wine-processed rhubarb and the processing mechanism” (BK2012349), July 2012-July 2015, ￥200,000.

2. Academic Awards

Third Prize of Teaching Competition for Young Teachers of National Medical Colleges and Universities.(2015)

Honorable mention of National Universities Micro-Teaching Competition.(2015)

First Prize of Universities Micro-Teaching Competition of Jiangsu Province.(2015)

First Prize of Teaching Competition for Young Teachers of China Pharmaceutical University.(2011)

3. Representative Research Achievements

(1)In view of the characteristics that oral traditional Chinese medicines are easy to metabolize in the intestine and liver, the work of using in vitro intestinal flora and liver microsomal incubation system to study the differences in metabolic activities and dominant metabolites of TCMs was carried out. A strategy for identifying metabolite structural modification sites based on the changes of ultraviolet spectral signatures of prototype compounds and metabolites was proposed, which provided a solution to the problem that substitution sites could not be determined based on mass spectrometry characteristic fragment ions alone.

(2)A platform of in vivo analysis technology of drug-derived components of traditional Chinese medicines based on combined metabolic characteristics was proposed and successfully applied to the identification and characterization of metabolites in Chaihu.

1.Qiwei Liu, Yunwen Xue, Jingjing Liu, et al. Saikosaponins and the deglycosylated metabolites exert liver meridian guiding effect through PXR/CYP3A4 inhibition. Journal of Ethnopharmacology, 2021, 279:114344. doi: 10.1016/j.jep.2021.114344. IF: 5.195

2.Min Wang, Jie Xu, Yuxin Zhang, NaYang, Weihong Ge*, Rui Song*.  Integrated multiplatform-based metabonomics and network analysis to explore the mechanism of Polygonum cuspidatum on hyperlipidemia. Journal of Chromatography B, 2021, 1176:122769. IF: 3.318

3.Siqi Ren, Jingjing Liu, Yunwen Xue, Mei Zhang, Qiwei Liu, Jie Xu, Zunjian Zhang, Rui Song*. Comparative permeability of three saikosaponins and corresponding saikogenins in Caco-2 model by a validated UHPLC-MS/MS method . Journal of Pharmaceutical Analysis, 2021 Aug;11(4):435-443. doi: 10.1016/j.jpha.2020.06.006. IF: 14.206

4.Zhang Yaqing, Zhang Mei, Hu Guangnan, Zhang Zunjian, Song Rui. Elevated system exposures of baicalin after combinatory oral administration of rhein and baicalin: Mainly related to breast cancer resistance protein (ABCG2), not UDP-glucuronosyltransferases. Journal of Ethnopharmacology, 2020, 250:112528. doi: 10.1016/j.jep.2019.112528. IF:4.360

5.Liu Jingjing, Xue Yunwen, Sun Jianbo, Fu Ruijia, Ren Siqi, Zhang Zunjian, Song Rui. Pharmacokinetics and oral bioavailability studies of three saikogenins in rats using a validated UFLC-MS/MS method. Journal of Chromatography B, 2019 Aug 15;1124:265-272. doi: 10.1016/j.jchromb.2019.06.020. IF: 3.004

6.Xue Yunwen, Liu Jingjing, Fu Ruijia, Gao Lei, Yang Xuping, Zhang Zunjian, Song Rui. In vitro studies on the metabolism of saikogenins and the detection of their metabolites in authentic biosamples. Journal of Pharmaceutical and Biomedical Analysis, 2019 Aug,172:295-301. doi: 10.1016/j.jpba.2019.04.051. IF:3.209