1. Research Projects
(1)Open ScientificProgram of Key Laboratory of Advanced Materials of Ministry of Education (ADV22-3): Aptamer- nanosized graphene hybrid Fluorescent platform: rapid fluorescence detection and drug screening of COVID-19
(2)National Natural Science Foundation of China (Grant No.21350161): Preparation and exploration of fluorescent grapheme dots and detection of hydroxyl radicals
(3)Natural Science Foundation of Jiangsu Province (Grant No. BK20130643): Antenna type graphene quantum dot immunofluorescence probe for detection of liver cancer marker alpha fetoprotein
(4)The Key Program of Fundamental Research Funds for the Central Universities (Grant No. 2632019ZD18): Exploration of enzyme-MOFs hybrid nanoreactor in the Directional drug synthesis
2. Academic Awards
(1)2010 Two research papers honored by top-50 citations publication in Analytica Chimica Acta (2005-2009)
(2)2011 Outstanding Doctoral Dissertation Award of Naikai University 2011
3. Representative Research Achievements
Representative Research Achievements 1#
Sulfur-containing species (SCS), especially sulfur dioxide-relevant species, play an essential role in ecological balance. Owing to the intrinsically labile and mobile characteristics of SCS, it is still considered to be an insurmountable challenge for multiplexed tracking dynamics of SCS with distinct molecular structure, valence state, and condensed state. To address this key problem, we proposed herein alternative versatile single-molecule sensors (VSMs) that intramolecularly integrate high affinity target-guided multiple recognition units into a single sensory molecule, clarified as molecular Nezha available in triplexed responses to gaseous sulfur dioxide, liquid sulfur trioxide, and aqueous bisulfite through ubiquitous charge transfer and nucleophilic addition. High-performance molecular Nezha remarkably facilitated promising applications in a quantitative visualization of SCS on lab-on-paper and tracking the dynamics transformation of SCS as well comprehensive evaluation of multiphase adsorption science of SCS on an advanced Zeolitic imidazolate framework-8 (ZIF-8).
Representative Research Achievements 2#
Daily monitoring of blood glucose is of great importance for the treatment of diabetes mellitus. Herein, we present an ensemble glucometer with a sandwich structure formed by the spontaneous entrapment of glucose oxidase (GOD) onto manganese dioxide nanosheets (MnO2 NSs) via the hydrophobic effect and hydrogen bond interaction. Within the hybrid glucometer, the ultrathin MnO2 NSs act as an enzyme nanosupport and target-activated signal transducer. Trimodal self-indication by fluorescence (FL) and UV-absorbance (UV) and magnetic resonance signal (MRS) activation with glucose-specificity provides multiple response signals to glucose. Taking account of its operational simplicity and convenience, even being observable by the naked eye, a detection limit as low as 0.1 µM was obtained by using the ensemble glucometer in a colorimetric assay, whilst the precision for 11 replicated detections of 10 µM glucose was 3.5% (relative standard deviation, RSD). Notably, the value of the Michaelis–Menton constant of GOD involved the presented glucometer is estimated to be 0.051 mM, showing an exceptional enhanced enzymatic activity of free GOD measured by far. The designed glucometer, with its high sensitivity and simplicity highlighted, was capable of routine blood glucose monitoring for type-I diabetes mellitus in rats. Furthermore, the fully integrated platform can be readily generalized in principle for a number of biomarkers for point of care diagnostics in the future.
Our research interesting is focused on exploration of design and exploration functional and state of the art luminescent and fluorescence molecular probes and biosensor for disease-related biomarkers and bioimaging of biochemical process as well for pharmaceutical analysis environmental analysis and IVD.
1. Research Projects
(1)Open ScientificProgram of Key Laboratory of Advanced Materials of Ministry of Education (ADV22-3): Aptamer- nanosized graphene hybrid Fluorescent platform: rapid fluorescence detection and drug screening of COVID-19
(2)National Natural Science Foundation of China (Grant No.21350161): Preparation and exploration of fluorescent grapheme dots and detection of hydroxyl radicals
(3)Natural Science Foundation of Jiangsu Province (Grant No. BK20130643): Antenna type graphene quantum dot immunofluorescence probe for detection of liver cancer marker alpha fetoprotein
(4)The Key Program of Fundamental Research Funds for the Central Universities (Grant No. 2632019ZD18): Exploration of enzyme-MOFs hybrid nanoreactor in the Directional drug synthesis
2. Academic Awards
(1)2010 Two research papers honored by top-50 citations publication in Analytica Chimica Acta (2005-2009)
(2)2011 Outstanding Doctoral Dissertation Award of Naikai University 2011
3. Representative Research Achievements
Representative Research Achievements 1#
Sulfur-containing species (SCS), especially sulfur dioxide-relevant species, play an essential role in ecological balance. Owing to the intrinsically labile and mobile characteristics of SCS, it is still considered to be an insurmountable challenge for multiplexed tracking dynamics of SCS with distinct molecular structure, valence state, and condensed state. To address this key problem, we proposed herein alternative versatile single-molecule sensors (VSMs) that intramolecularly integrate high affinity target-guided multiple recognition units into a single sensory molecule, clarified as molecular Nezha available in triplexed responses to gaseous sulfur dioxide, liquid sulfur trioxide, and aqueous bisulfite through ubiquitous charge transfer and nucleophilic addition. High-performance molecular Nezha remarkably facilitated promising applications in a quantitative visualization of SCS on lab-on-paper and tracking the dynamics transformation of SCS as well comprehensive evaluation of multiphase adsorption science of SCS on an advanced Zeolitic imidazolate framework-8 (ZIF-8).
Representative Research Achievements 2#
Daily monitoring of blood glucose is of great importance for the treatment of diabetes mellitus. Herein, we present an ensemble glucometer with a sandwich structure formed by the spontaneous entrapment of glucose oxidase (GOD) onto manganese dioxide nanosheets (MnO2 NSs) via the hydrophobic effect and hydrogen bond interaction. Within the hybrid glucometer, the ultrathin MnO2 NSs act as an enzyme nanosupport and target-activated signal transducer. Trimodal self-indication by fluorescence (FL) and UV-absorbance (UV) and magnetic resonance signal (MRS) activation with glucose-specificity provides multiple response signals to glucose. Taking account of its operational simplicity and convenience, even being observable by the naked eye, a detection limit as low as 0.1 µM was obtained by using the ensemble glucometer in a colorimetric assay, whilst the precision for 11 replicated detections of 10 µM glucose was 3.5% (relative standard deviation, RSD). Notably, the value of the Michaelis–Menton constant of GOD involved the presented glucometer is estimated to be 0.051 mM, showing an exceptional enhanced enzymatic activity of free GOD measured by far. The designed glucometer, with its high sensitivity and simplicity highlighted, was capable of routine blood glucose monitoring for type-I diabetes mellitus in rats. Furthermore, the fully integrated platform can be readily generalized in principle for a number of biomarkers for point of care diagnostics in the future.
1. Wang, S.; Ren, J.-S.; Shan, L.-Y.; Sun, X.-Y.; Di, B.; Gu, N.; Chen, J.-L., Triplexed Tracking Labile Sulfur-Containing Species on a Single-Molecule “Nezha” Sensor. Analytical Chemistry 2020, 92 (3), 2672-2679. Impact Factor:6.755
2. Chen, J.-L.; Li, L.; Wang, S.; Sun, X.-Y.; Xiao, L.; Ren, J.-S.; Di, B.; Gu, N., A glucose-activatable trimodal glucometer self-assembled from glucose oxidase and MnO2 nanosheets for diabetes monitoring. Journal of Materials Chemistry B 2017, 5 (27), 5336-5344. Impact Factor: 4.9591
3. Qu, D.-Y.; Chen, J.-L.; Di, B., A fluorescence “switch-on” approach to detect hydrazine in aqueous solution at neutral pH. Analytical Methods 2014, 6 (13), 4705-4709. Impact Factor: 1.821
4. Yan, F.; Liu, Z.-Y.; Chen, J.-L.; Sun, X.-Y.; Li, X.-J.; Su, M.-X.; Li, B.; Di, B., Nanoscale zeolitic imidazolate framework-8 as a selective adsorbent for theophylline over caffeine and diprophylline. RSC Advances 2014, 4 (62), 33047-33054. Impact Factor: 3.840
5. Chen, J.-L.; Yan, X.-P.; Meng, K.; Wang, S.-F., Graphene Oxide Based Photoinduced Charge Transfer Label-Free Near-Infrared Fluorescent Biosensor for Dopamine. Analytical Chemistry 2011, 83 (22), 8787-8793. Impact Factor: 5.35
6. Chen, J.-L.; Yan, X.-P., Ionic strength and pH reversible response of visible and near-infrared fluorescence of graphene oxide nanosheets for monitoring the extracellular pH. Chemical Communications 2011, 47 (11), 3135-3137. Impact Factor: 6.287
7. Chen, J.-L.; Yan, X.-P., A dehydration and stabilizer-free approach to production of stable water dispersions of graphene nanosheets. Journal of Materials Chemistry 2010, 20 (21), 4328-4332. Impact Factor: 4.795
8. Zhu, C.-Q.; Chen, J.-L.; Zheng, H.; Wu, Y.-Q.; Xu, J.-G., A colorimetric method for fluoride determination in aqueous samples based on the hydroxyl deprotection reaction of a cyanine dye. Analytica Chimica Acta 2005, 539 (1), 311-316. Impact Factor: 2.914
9. Chen, J. L.; Zhu, C. Q., Functionalized cadmium sulfide quantum dots as fluorescence probe for silver ion determination. Analytica Chimica Acta 2005, 546 (2), 147-153. Impact Factor: 2.914
10. Zhu, C. Q.; Zhao, D. H.; Chen, J. L.; Li, Y. X.; Wang, L. Y.; Wang, L.; Zhou, Y. Y.; Zhuo, S. J.; Wu, Y. Q., Application of l-cysteine-capped nano-ZnS as a fluorescence probe for the determination of proteins. Analytical and Bioanalytical Chemistry 2004, 378 (3), 811-815. Impact Factor: 2.098
