1. Nanobiosensors, mainly focus on fluorescent biosensor and lateral immunochromatography, which would be applied for rapid detection of drugs, toxins and microorganisms in food or drug.

2. Multifuntional nanoprobes for disease diagnostics, achieve accurate diagnosis and imaging-guided treatment of different types of cancer and antibacterial therapy.

3. Microneedles, develop percutaneous delivery system to achieve painless, no bleeding and high efficient delivery of drugs in animal model, such as diabetes, high blood pressure, and contraception.

1. Research Projects

(1)National Natural Science Foundation of China,Period:2021.01-2023.12,Grant Number:No. 52002402,240 thousand RMB

(2)Natural Science Foundation of Jiangsu Province,Period:2020.07-2023.06,Grant Number:No. BK20200574,20 thousand RMB

(3)Doctor of Entrepreneurship and Innovation in Jiangsu Province,Period:2020.09-2022.09,Grant Number:---,15 thousand RMB

(4)National Key Research and Development Program,Period:2019.12-2022.12,Grant Number:2019YFC1605400,532.4 thousand RMB

(5)Initial Project for High-level Talent of China Pharmaceutical University,Period:2019.09-2025.08,Grant Number:---,1 million RMB

2. Representative Research Achievements

(1)In traditional lateral flow immunoassays (LFIA) for pathogens detection, capture antibody (CA) is necessary and usually conjugated to Au nanoparticles (NPs) in order to label the target analyte. However, the acquisition process of the Au−CA nanoprobe is relatively complicated and costly, which will limit the application of LFIA. Herein, p-mercaptophenylboronic acid-modified Au NPs (namely Au−PMBA nanocrabs), were synthesized and applied for a new CA-independent LFIA method. This CA-independent strategy exhibited higher sensitivity than the traditional CA-dependent double antibody sandwich method, because detection limit of the former one was 10³ cfu/mL only by visual observation, which was reduced by 3 orders of magnitude. This CA-independent LFIA showed great advantages and satisfactory potential for rapid foodborne pathogens detection in real sample.

(2)Bacterial infections with a high mortality rate have become serious health issues for human beings. Current treatment remains unsatisfactory due to the limited antibacterial efficacy triggered by weak tissue and biofilm permeability of drugs and the risk of antibiotic resistance. To address these issues, a novel enzymatic antibacterial strategy, is developed with highly peroxidase-like activities. Both in vitro and in vivo experiments exhibit superior synergistic antibacterial efficacy. The antimicrobial mechanisms are explained as the reduction of natural enzyme activities and the disruption of cell walls and membranes along with the production of abundant •OH radicals derived from nanozymes, which provides a potential platform for future synergistic antibacterial application.

1. Wu, P. C.; Zuo, W. C.; Wang, Y. F.; Yuan, Q. F.; Yang, J.; Liu, X. M.; Jiang, H.; Dai, J. J.; Xue, F.; Ju, Y. M. Multimodal capture-antibody-independent lateral flow immunoassay based on AuNF-PMBA for point-of-care diagnosis of bacterial urinary tract infections. Chemical Engineering Journal 2023, 451, 11. (IF=16.744)

2. Zuo, W. C.; Wu, P. C.; He, W.; Xiao, Q. Q.; Yang, J.; Liu, X. M.; Jiang, H.; Dai, J. J.; Ju, Y. M. A fluorescent and ratiometric colorimetric biosensor for detection of different hazard contaminants in dairy products. Sensors and Actuators: B. Chemical 2023, 374, 9. (IF=9.221)

3. Sun, C. X.; Wang, X. B.; Dai, J. J.; Ju, Y. M. Metal and Metal Oxide Nanomaterials for Fighting Planktonic Bacteria and Biofilms: A Review Emphasizing on Mechanistic Aspects. International Journal of Molecular Sciences 2022, 23 (19), 22. (IF=6.208)

4. Wu, P. C.; Xue, F.; Zuo, W. C.; Yang, J.; Liu, X. M.; Jiang, H.; Dai, J. J.; Ju, Y. M. A Universal Bacterial Catcher Au-PMBA-Nanocrab-Based Lateral Flow Immunoassay for Rapid Pathogens Detection. Analytical Chemistry 2022, 94 (10), 4277-4285. (IF=8.008)

5. Ju, Y. M.; Wang, Z. Y.; Ali, Z. S.; Zhang, H. C.; Wang, Y. Z.; Xu, N.; Yin, H.; Sheng, F. G.; Hou, Y. L. A pH-responsive biomimetic drug delivery nanosystem for targeted chemo-photothermal therapy of tumors. Nano Research 2022, 15 (5), 4274-4284. (IF=10.269)

6. Sun, C. X.; Wang, W. Q.; Sun, X. L.; Chu, W. H.; Yang, J.; Dai, J. J.; Ju, Y. M. An intrinsically thermogenic nanozyme for synergistic antibacterial therapy. Biomaterials Science 2021, 9 (24), 8323-8334. (IF=7.59)

7. Chen, W. T.; Zhang, F. F.; Ju, Y. M.; Hong, J.; Ding, Y. Gold Nanomaterial Engineering for Macrophage-Mediated Inflammation and Tumor Treatment. Advanced Healthcare Materials 2021, 10 (5). (IF=9.93)

8. Ju, Y. M.; Dong, B.; Yu, J.; Hou, Y. L. Inherent multifunctional inorganic nanomaterials for imaging-guided cancer therapy. Nano Today 2019, 26, 108-122. (IF=16.907)

9. Ju, Y. M.; Zhang, H. L.; Yu, J.; Tong, S. Y.; Tian, N.; Wang, Z. Y.; Wang, X. B.; Su, X. T.; Chu, X.; Lin, J.; et al. Monodisperse Au-Fe₂C Janus Nanoparticles: An Attractive Multifunctional Material for Triple-Modal Imaging-Guided Tumor Photothermal Therapy. ACS Nano 2017, 11 (9), 9239-9248. (IF=14.357)

10. Wang, Z. Y.; Ju, Y. M.; Ali, Z.; Yin, H.; Sheng, F. G.; Lin, J.; Wang, B. D.; Hou, Y. L.  Near-infrared light and tumor microenvironment dual responsive size-switchable nanocapsules for multimodal tumor theranostics. Nature Communications 2019, 10 (1), 4418-4418. (IF=11.878)

11. Dong, B.; Ju, Y. M.; Huang, X. X.; Li, W.; Ali, Z. S.; Yin, H.; Sheng, F. G.; Hou, Y. L. A general strategy for facile synthesis of ultrathin transition metal hydroxide nanosheets. Nanoscale 2019, 11 (12), 5141-5144. (IF=6.97)

12. Wang, Z. Y.; Ju, Y. M.; Tong, S. Y.; Zhang, H. C.; Lin, J.; Wang, B. D.; Hou, Y. L. Au₃Cu tetrapod nanocrystals: highly efficient and metabolizable multimodality imaging-guided NIR-II photothermal agents. Nanoscale Horizons 2018, 3 (6). (IF=9.095)